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Quantitative and Functional Posttranslational Modification Proteomics Reveals that TREPH1 Plays a Role in Plant Touch-delayed Bolting

Penelitian - Researchers suggest protein phosphorylation and the touch-regulated phosphoprotein1 (TREPH1) protein are critical for the mechanotransduction pathway leading to an aspect of plant thigmomorphogenesis.

Environmental mechanical forces, such as wind and touch, trigger gene-expression regulation and developmental changes, called “thigmomorphogenesis” in plants, demonstrating the ability of plants to perceive such stimuli.

Penelitian Quantitative and Functional Posttranslational Modification Proteomics Reveals that TREPH1 Plays a Role in Plant Touch-delayed Bolting

In Arabidopsis, a major thigmomorphogenetic response is delayed bolting. The signaling components responsible for mechanotransduction of the touch response are largely unknown.

Kai Wang of the The Hong Kong University of Science and Technology in China and team performed a high-throughput SILIA (stable isotope labeling in Arabidopsis)-based quantitative phosphoproteomics analysis to profile changes in protein phosphorylation resulting from 40 seconds of force stimulation in Arabidopsis thaliana.

Of the 24 touch-responsive phosphopeptides identified, many were derived from kinases, phosphatases, cytoskeleton proteins, membrane proteins, and ion transporters. In addition, the previously uncharacterized protein TREPH1 became rapidly phosphorylated in touch-stimulated plants, as confirmed by immunoblots.

A nonphosphorylatable site-specific isoform of TREPH1 (S625A) failed to restore touch-induced flowering delay of treph1-1, indicating the necessity of S625 for TREPH1 function and providing evidence consistent with the possible functional relevance of the touch-regulated TREPH1 phosphorylation.



“These findings identify a phosphoprotein player in Arabidopsis thigmomorphogenesis regulation and provide evidence that TREPH1 and its touch-induced phosphorylation may play a role in touch-induced bolting delay, a major component of thigmomorphogenesis,” Wang said.

Journal : Kai Wang et al. Quantitative and functional posttranslational modification proteomics reveals that TREPH1 plays a role in plant touch-delayed bolting, PNAS, October 5, 2018, DOI:10.1073/pnas.1814006115

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