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Accelerated Ex situ Breeding of GBSS- and PTST1-edited Cassava for Modified Starch

Penelitian - Scientists have recently succeeded in using gene-editing technology to develop a variant of the starchy tuber cassava that is preferred by the food and paper industries. Cassava is one of the world's most important starch crops.

Although the plants are hardy and can survive even in drought conditions, which makes it one of the top five most important sources of carbohydrate globally, it's time-consuming to cross-breed useful new traits into different farmer-preferred varieties.

Penelitian Accelerated Ex situ Breeding of GBSS- and PTST1-edited Cassava for Modified Starch

In a study recently published on the Science Advances, researchers at the Swiss Federal Institute of Technology (ETH Zurich) opted to take a new approach to introduce new traits into cassava.

They used the famous CRISPR-Cas9 gene editing tool, dubbed gene scissors, to change two cassava genes so that the plant produces modified starch that has little or no amylose, and is in high demand on the global market.

To achieve this, the researchers inserted a block of several foreign genes into the cassava plants. This block included the genes for the Cas9 protein and for the guide RNA molecule that the CRISPR-Cas9 system needs in order to cut the genetic material at the desired point.

It also contained a gene from another plant, called the thale cress Arabidopsis, to accelerate flowering. As starch is composed of about 85 percent of amylopectin and 15 percent of amylose, the latter being the cause that makes processing the plant for food very difficult, the new variant turned out to be much easier to process commercially.

Meanwhile, more frequent flowering made it easier to breed the new, amylose-free variety much faster, and to produce offspring that did not contain any foreign Arabidopsis genes.

For that reason, the industry would be particularly interested in amylose-free cassava starch, as removal of amylose usually requires more processing and energy-consuming methods for starch purification. Also, consumers may also prefer the waxy, amylose-free starch which is an important source of carbohydrates particularly in the southern hemisphere and in African countries.

Journal : Simon E. Bull et al. Accelerated ex situ breeding of GBSS- and PTST1-edited cassava for modified starch, Science Advances, 05 Sep 2018, DOI:10.1126/sciadv.aat6086

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