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Human organoids to predict in vitro activity of each flu strain

Penelitian - Influenza virus infection is a continuing threat to public health worldwide, but to date there are no biologically relevant models to be reproduced and available in vitro to predict infectivity in humans. Scientists at the University of Hong Kong developed the proximal differentiation and 2D monolayer culture of the 3D human airway organ.

Novel reassortant avian influenza H7N9 virus and pandemic 2009 H1N1 (H1N1pdm) virus cause human infection, while avian H7N2 and swine H1N1 virus only kill on birds and pigs. The problem becomes prolonged because there is no strong in vitro model to assess the viral infectivity that appears in humans.

Penelitian Human organoids to predict in vitro activity of each flu strain

Kwok-Yung Yuen from the University of Hong Kong and colleagues built a 3D human airway organ accommodating four different types of airway epithelial cells are ciliated, goblet, club, and basal cells to analyze the differentiation that increases the number of cells in each organoid. Differentiation also induces an increase in serine proteases levels that are important in productive infections in both low pathogenic human and bird flu viruses.

3D and 2D airway organelles demonstrate a differentiated airway to identify viruses that infect humans to evaluate two pairs of viruses with known infectivities that are different in humans and are applied for rapid assessment of infectious respiratory viruses that appear to humans.



Proximal organisms can morphologically and functionally simulate the human airway epithelium and as proof of concept to distinguish influenza virus. Thus, differentiated airway organ can be used to predict infectivity of the virus and provide a universal platform for the biology and pathology of the human airway.

Journal : Jie Zhou et al. Differentiated human airway organoids to assess infectivity of emerging influenza virus, PNAS, June 11, 2018, DOI:10.1073/pnas.1806308115

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